High-resolution cryo-EM structure reveals the AUT5 binding site in Kv3.1. a Overall cryo-EM reconstruction of Kv3.1/AUT5 complex. EM map feature for AUT5 is highlighted in red. b Cartoon representation of AUT5-binding site. Key residues for interaction with AUT5 (dark red) are highlighted as stick representations. Hydrogen bonds and van der Waals contacts are shown as black and orange dashed lines, respectively. The black dashed lines indicate potential polar interactions within a distance ?3.2?Å. c, d Cartoon representations of conformational change of AUT5-binding site upon compound binding. Compared to Kv3.1 in Read More