Evaluation of TH17 profile in common variable immunodeficiency patients with or without autoimmunity

Forty two CVID patients were recruited from the PID outpatient clinic, Clinical Immunology
and Allergy Division of HC-FMUSP, being 17 with and 25 without autoimmunity. T lymphocyte
characterization was done by flow cytometry using the following panels: activation
panel (CD3, CD4, CD8, HLA-DR, CD38, CD69, Live/dead); regulatory T cells panel (CD3, CD4, CD8, CD25, CD39, CD45-RO, CD127, Live/dead, FoxP3); and functional panel – upon 5 hours stimulation – (CD3, CD4, CD8, Live/dead, TNF-?, IFN-?_, IL-2, IL-17, IL-21).