Table 1
High-level categories (indicated by stars) and characters implemented in MicroPIE
Categories*/characters
Example source sentences
G?+?C Content *
%G?+?C
DNA G?+?C content is 33.1–34.4 mol% .
Cell Morphology *
Cell Shape
Cells are slender, cylindrical, sometimes crooked rods that are 0.35–0.5 ?m wide and 2.5 ?m long and occur singly or in pairs, or in longer chains.
Cell Diameter
Cells are 0.3–0.5 ?m in diameter.
Cell Length
In glucose broth, the bacilli are longer, up to 4 ?m long, and often occur in pairs.
Cell Width
In addition, cells have an outer diameter of 1.5–3.0 ?m and width of 0.5–0.9 ?m .
Cell Relationship Aggregations
A few strains grow in chains of 3 to 6 elements in glucose broth.
Gram Stain Type
The cells are Gram-negative , long, slender rods or sometimes filaments, motile by gliding.
External features
Cells are frequently occurring in chains and producing appendages in older cultures.
Internal features
No spores are observed.
Motility
Cells are non-motile , extremely pleomorphic
Pigment Compounds
Pigmented red due to carotenoids.
Growth Condition *
NaCl Minimum
Growth requires at least 1.7 M NaCl, optimally 3.0–4.3 M NaCl .
NaCl Optimum
NaCl Maximum
pH Minimum
Growth occurs at temperatures in the range 38–68 °C (optimum, 60 °C ) and at pH 1.8–4.0 (optimum, pH 3.0 ).
pH Optimum
pH Maximum
Temperature Minimum
Temperature Optimum
Temperature Maximum
Salinity Requirement for Growth
Requires sea salts for growth.
Aerophilicity
Strictly anaerobic. Obligately anaerobic.
Magnesium Requirement for Growth
Mg2+ and Ca2+ enhance growth.
Vitamins and Cofactors Used For Growth
Vitamin K1 and haemin are required for growth.
Antibiotic Physiology *
Antibiotic Sensitivity
Sensitive to (?g per disc) tetracycline (30), rifampicin (25) and vancomycin (30), but resistant to ampicillin (25), erythromycin (15), streptomycin (10) and gentamicin (10).
Antibiotic Resistant
The type strain is resistant to erythromycin .
Colony Morphology*
Colony Shape
On MA, colonies are convex , translucent, shiny and smooth.
Colony Margin
Colonies are golden-yellow, circular and convex, with an entire edge on MA.
Colony Texture
On MA, colonies are convex, translucent, shiny and smooth .
Colony Color
Colonies are golden-yellow , circular and convex, with an entire edge and a butyrous consistency on MA.
Metabolic End Products and Secondary Metabolites*
Fermentation Products
Acid is produced from glucose and fructose.
Other Metabolic Products
Acetoin is produced.
Microbe-Host Interaction*
Pathogenic
Pathogenic for humans , causing wound infections.
Disease Caused
Pathogenic for honeybees in natural and experimental oral infections .
Pathogen Target Organ
Nodosus is infected hooves of sheep and goats.
Haemolytic/Haemadsorption Properties
No hemolysis occurs on rabbit blood agar.
Metabolic Substrate*
Organic Compounds Used Or Hydrolyzed
Utilize carbohydrates for growth.
Organic Compounds Not Used Or Not Hydrolyzed
Arabinose, mannose, N-acetylglucosamine, maltose are used as sole carbon and energy source but not mannitol, gluconate, caprate, adipate, citrate and malate .
Inorganic Substances Used
Does not require yeast extract for growth, and can use inorganic nitrogen sources such as sodium nitrate and ammonium chloride .
Inorganic Substances Not Used
Does not require yeast extract for growth, and can use inorganic nitrogen sources such as sodium nitrate and ammonium chloride.
Fermentation Substrates Used
Ferments proteinaceous compounds .
Fermentation Substrates Not Used
And no acid is produced from glycerol, erythritol, L-arabinose, D-ribose, or potassium 5-ketogluconate .
Example source sentences for each character within each category are provided. Bolded text in the source sentences indicates the values that MicroPIE should extract