Relevance of IgE to novel kiwi seed allergens evaluated in kiwi allergic children

Kiwi seeds were isolated, extracted and analyzed using chromatographic methods, MALDI-TOF
and SDS-PAGE. Patient samples from children with documented primary kiwi allergy were
used for evaluation. Care was taken to not include patients primarily sensitized to
birch pollen in this study, to avoid cross-reactivity between Bet v 1 and the homologous
Act d 8. IgE-immunoreactivity towards all four purified proteins, as well as the whole
kiwi seed extract, was studied.