Xylanase gene isolated from Bacillus brevis was expressed in E. coli BL21.
Sequencing of the gene (Gen Bank accession number: HQ179986) showed that it belongs to family 11 xylanases. The recombinant xylanase was predominantly secreted to culture medium and showed mesophilic nature (optimum activity at 55[degree sign]C and pH 7.0).
The cell free culture medium exhibited 30 IU/ml xylanse activity. The enzyme did not show any cellulose activity and was active under wide range of temperature (40[degree sign]C to 80[degree sign]C) and pH (4 to 9).
The enzyme showed considerable thermo stability and regained over 90% of activity, when returned to 55[degree sign]C after boiling for 5 min. These physiochemical properties of B.
brevis xylanse show high potential of its applications in paper and pulp industry.
Author: Girish K GoswamiMedichtrla KrishnamohanVikrant NainChetana AggarwalBandarupalli Ramesh
Credits/Source: SpringerPlus 2014, 3:20
Published on: 2014-01-10
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