healthmedicinet

In 18/36 patients tested drugs elicited granzyme B release in ELISpot. Staining for
granulysin was positive in 15/36 patients, whereas increased secretion of perforin
was detected in 3/36 patients. 11/36 patients showed positive proliferative response
to the drug, measured in lymphocyte transformation test. None of the 24 drug-exposed
healthy donors reacted to the tested drugs in perforin assay and LTT, however one
reacted in GrB-ELISpot and another one in granulysin assay. Obtained data correspond
to the following sensitivities and specificities, respectively: GrB-ELISpot: 53% and
96%; Grl-NK: 44% and 96%; Per-ELISA: 9% and 100%; LTT: 32% and 100%.