Helicobacter pylori, a gram-negative bacterial pathogen that expresses a strong urease activity, is associated with the development of gastroduodenal disease. Urease B subunit, one of the two structural subunits of urease, was expressed in E.
coli BL21 (DE3) strain. The objective of this study was to evaluate the effects of Helicobacter pylori urease B subunit on the immune responses in mice by subcutaneous immunization.
Methods:
The mice were immunized and boosted with Helicobacter pylori urease B subunit antigen subcutaneously three times with 2-week intervals between the immunizations and boosters.
The mice in the control group were immunized with PBS. The adjuvant group received PBS containing complete/incomplete freund’s adjuvant identical to antigen group without Helicobacter pylori urease B subunit antigen.
Four weeks after the final booster, all the mice were sacrificed. Blood was collected on d 0, 14, 28 and 56 before immunization, booster and sacrifice, respectively.
Immediately after sacrifice, gastric liquid and spleen were collected for antibody and cytokine analyses.
Results:
Urease B subunit increased the concentrations of serum and gastric anti-urease B antigen specific IgG, and the levels of interleukin-4 and interferon-gamma in splenocytes of the mice (P
Conclusions:
This study demonstrated that recombinant urease B subunit can induce systemic and local immune responses in mice by subcutaneous immunization, which might be used as the effective component of vaccine against Helicobacter pylori.
Author: Peng SunJia-Qi WangYu-Tao ZhangSheng-Guo Zhao
Credits/Source: Journal of Animal Science and Biotechnology 2014, 5:14
Published on: 2014-02-22
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News Provider: EUPB – European Press Bureau
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