Cryopreservation of the ovarian tissue has shown promising results. However, there remain controversial issues such as the short half-life of grafts.
In this aspect, there are some evidences that preconditioning the ovarian tissue before transplantation is beneficial.ObjectiveTo determine the effect of hypoxic preconditioning in vitro on ovarian tissue prior to transplantation.
Methods:
Eighteen female adult Wistar rats, were sorted into three experimental groups. Ovaries were maintained in DMEM low glucose serum free at 37[degree sign]C with 5% CO2, at atmospheric oxigen concentration (normoxia) or 1% O2 (hypoxia) for 16 hours.
Oxigen concentration was determined by injection of nitrogen in the incubator. Animals submitted to ovarian transplantation immediately after oophorectomy were the Control Group (C).
After this, the ovaries were implanted in the retroperitoneum with nonabsorbable suture and animals evaluated for thirty days after transplantation. Beginning on postoperative (PO) day 11, a daily collection of vaginal smear was carried out.
Analyses comprised morphological, morphometric (counting ovarian follicles and corpora lutea) and immunohistochemistry for cleaved caspase-3 (apoptosis).
Results:
In normoxia and control groups all animals recovered their estrous cycles, while in the hypoxia group, two animals did not ovulate but, among those which did, resumption took longer than in the other groups (p
Conclusion:
The hypoxic preconditioning in vitro was not beneficial to the graft and worsened their viability, compromising its functionality or delaying the return of this.
Author: Luciana Lamarão DamousJuliana Sanajotti NakamutaJosé Maria Soares-JrGustavo Arantes MacielRicardo dos SimõesEdna Frasson MonteroJosé Eduardo KriegerEdmund Chada Baracat
Credits/Source: Journal of Ovarian Research 2014, 7:34
Published on: 2014-03-20
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