Plasmodium knowlesi is the fifth Plasmodium species that can infect humans. The Plasmodium merozoite surface protein-142 (MSP-142) is a potential candidate for malaria vaccine.
However, limited studies have focused on P. knowlesi MSP-142.
Methods:
A ~42 kDa recombinant P.
knowlesi MSP-142 (pkMSP-142) was expressed using an Escherichia coli system. The purified pkMSP-142 was evaluated with malaria and non-malaria human patient sera (n = 189) using Western blots and ELISA.
The immunogenicity of pkMSP-142 was evaluated in mouse model.
Results:
The purified pkMSP-142 had a sensitivity of 91.0% for detection of human malaria in both assays. Specificity was 97.5 and 92.6% in Western blots and ELISA, respectively.
Levels of cytokine interferon-gamma, interleukin-2, interleukin-4, and interleukin-10 significantly increased in pkMSP-142-immunized mice as compared to the negative control mice. pkMSP-142-raised antibody had high endpoint titres, and the IgG isotype distribution was IgG1 IgG2b IgG3 IgG2a.
Conclusions:
pkMSP-142 was highly immunogenic and able to detect human malaria.
Hence, pkMSP-142 would be a useful candidate for malaria vaccine development and seroprevalence studies.
Author: Fei Wen CheongMun Yik FongYee Ling LauRohela Mahmud
Credits/Source: Malaria Journal 2013, 12:454
Published on: 2013-12-19
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News Provider: EUPB – European Press Bureau
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