Background}Contradictory evidence has been published on the effects of steroid treatments on the outcomes ofkidney and liver transplantation from brain dead (BD) donors. Our study aimed to evaluate thisdisparity by investigating the effect of prednisolone administration on BD rats.
Methods:
BD induction was performed in ventilated rats by inflating a Fogarty catheter placed in the epiduralspace.
Prednisolone (22.5mg/kg) was administered 30 min prior to BD induction. After four hours ofdetermination of BD: serum, kidney and liver tissues samples were collected and stored.
RT-qPCR,routine biochemistry and immunohistochemistry were performed.
Results:
Prednisolone treatment reduced circulating IL-6 and creatinine plasma levels but not serum AST, ALTor LDH. Polymorphonuclear influx assessed by histology, and inflammatory gene expression werereduced in the kidney and liver.
However, complement component 3 (C3) expression was decreasedin kidney but not in liver. Gene expression of HSP-70, a cytoprotective protein, was down-regulatedin the liver after treatment.
Conclusions:
This study shows that prednisolone decreases inflammation and improves renal function, whilst notreducing liver injury.
The persistence of complement activation and the negative effect on protectivecellular mechanisms in the liver may explain the disparity between the effects of prednisolone on thekidney and liver of BD rats. The difference in the molecular and cellular responses to prednisoloneadministration may explain the contradictory evidence of the effects of prednisolone on different organtypes from brain dead organ donors.
Author: Rolando RebolledoBo LiuMohammed Z AkhtarPetra J OttensJian-ning ZhangRutger J PloegHenri GD Leuvenink
Credits/Source: Journal of Translational Medicine 2014, 12:111
Published on: 2014-05-03
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