Washington State University researchers have found that the timing of an animalâ€™s sleep can be just as important as how much sleeps it gets.
Ilia Karatsoreos, an assistant professor in WSUâ€™s Department of Integrative Physiology and Neuroscience, shifted mice from their usual cycle of sleeping and waking and saw that, while they got enough sleep, it was of poorer quality. The animals also had a disrupted immune response, leaving them more open to illness.
Most sleep research focuses on the effects of sleep deprivation or the overall amount of sleep an animal needs. This is generally referred to as sleepâ€™s homeostatic process, which is driven by sleepiness or â€œsleep pressure.â€
The work by Karatsoreos and his colleaguesâ€”published in the journal Brain, Behavior and Immunityâ€”is a rare look into the circadian process, a brain-driven clock that controls the rhythms of various biological processes, from digestion to blood pressure, heart rate to waking and sleeping. The cycle is found in most everything that lives more than 24 hours, including plants and single-celled organisms.
Research into the system has significant implications for modern living, write Karatsoreos and his coauthors, as â€œdisruption of the circadian clock is nearly ubiquitous in our modern societyâ€ due to nighttime lighting, shift work, jet lag and even the blue-tinged light emitted by cell phones and tablets.
Typically, sleep researchers have a hard time studying sleep deprivation and the circadian cycle separately, as a change in one usually affects the other. However, Karatsoreos and his colleagues saw their model did not affect an animalâ€™s total sleep, giving them a unique look into the effects on the timing of the sleeping-waking cycle.
The researchers used mice whose body clocks run at about 24 hours â€“ much like our own â€“ and housed them in a shorter 20-hour day. This forced their biological clocks out of sync with the light-dark cycle. After four weeks, the researchers injected the mice with lipopolysaccharide, a molecule found in bacteria that can make an animal sick without being contagious.
The researchers saw that the disrupted animals had blunted immune responses in some cases or an overactive response in others, suggesting the altered circadian cycle made them potentially less able to fight illness and more likely to get sick.
â€œThis represents a very clear dysregulation of the system,â€ said Karatsoreos. â€œThe system is not responding in the optimal manner.â€Over time, he said, this could have serious consequences for an organismâ€™s health.
â€œJust like you have a car that youâ€™re running into the groundâ€”things donâ€™t work right but you keep driving it until it stops. Thatâ€™s what could happen if you think of disruption going on for years for somebody whoâ€™s working shift work,â€ he said.
To his surprise, the mice on the 20-hour cycle were getting the same amount of sleep as they did on the 24-hour cycle. But the sleep wasnâ€™t as good. The mice woke more often and the pattern of electrical activity in their brains related to restorative sleep was greatly reduced.
Adjusting your body clock when the time changes